Concentrated Tris-Taurine-EDTA buffer solution in distilled/deionized water. TTE (20X) eliminates the band distortion associated with DNA samples in glycerol. National Diagnostics' TTE Glycerol Tolerant Buffer (20X) is supplied in 1 liter bottles, containing 1.78M Tris base, 0.57M Taurine, and 10mM Na2EDTA.